Finally I finished writing a small report (in danish) about the old peas in the laboratory.
The report is located here: 2010 Resultat af ærteforsøg (DNA)
I had google translate this version, with a bit of my help: 2010 Pea results
When reading the results, so it is vital to understand that this investigation can determine whether some of the peas are genetically different, but can not determine whether they are genetically identical! We have investigated only some small bits of the total DNA.
The peas that look similar, are either identical or different, we do not know!
At the top is shown the principal coordinate analysis. It gives a good visual picture of the distance between our varieties. Some old Swedish varieties was blended in, as they have been studied previously. It gives some reference points to past studies.
The principal coordinate analysis is based of course, on some very concrete data, namely the length of the micro-satellites we tested.
Micro-satellites we have measured have letter names. A9 – D21 – AC58 – C20 – AA5 – AC75.
We investigated 6 different, but one would not work, the second was identical in all varieties, and therefore uninteresting. The last four showed differences. Each micro-satellite was measured twice.
I end up concluding:
So there are varieties that we now know is different from all others in this sample.
There are also groups of varieties we should check for phenotype differences. It is a great advantage that it is now clearly delineated the varieties to be compared by cultivation experiments. There is probably also varieties that are identical with each other. It is not something that can be resolved by this trial, which is only suitable to detect differences in the 4 areas (micro-satellites), we got useful results from. Whether they are identical in many other micro-satellites, we can not know before we make a new attempt!
Read in the google translated report: 2010 Pea results
If you can take a bit more, this is the excel file we were sent after the course: Diversity analysis Pisum