the world around



Baked pumpkin with Jerusalem artichoke, pepper, pee shoots and lemongrass
© Randi and Svend Sørensen

Food workshop – Samples and recipes with Danish Seed Savers own varieties. Part of Danish Seed Savers spring course, medio March.

Early in the winter I was asked to do a food workshop in March. Almost all crops was harvested and eaten by then. It was too late to sow new crops. Or so I thought. It turned out that I had time to cultivate pea shoots. And winter was mild, so there were left over small turnips in the garden, though the best was eaten. And while the focus was on seed saver cultivars, wild plants could also be included. And some fellow seed savers offered some of their crops for the day. So even in mid-March it was possible to present a wide range of flavours and cooking methods.

The samples was organised in stations. Clever women helped me set up the stations early in the morning. Each station should present a little harmony, but the order of stations should also guide taste buds through a landscape of sour, salty, bitter, umami and sweet. I had used very little spices, at the cultivars taste should be brought out. I had no intentions, that participants will copy this at home, but I hope the will feel empowered to explore old cultivars and wild edibles at home. Delicious cultivars are easy to preserve for future. Cultivars you don’t like to eat tend to be a pain to keep in culture. But often the difference is in the preparation.
The little old Errindlev pea was only four seeds from extinction. It was presented to the seed savers as an almost inedible, but old cultivar. I had been grown by an old woman in a village. She presented the seeds to younger people, but somehow her knowledge on the cultivar didn’t follow the seeds. Indeed, eaten as a green pea, it is no pleasure at all. But as a snow-pea and as a dry grey pea it is gourmet food. It almost became extinct before proper uses was discovered. Hereafter I listen more carefully to old people when they hand over their unique cultivars.

 

 


Salad of dandelion and turnip
©Randi og Svend Sørensen

Station 1
Salad of dandelion root and leaves, turnip and ramsons, with a pine oil and verjuice dressing

My thought was that it’s good to start with a salad. I have experience with this combination, where dandelion sweetness, nuttyness and bitterness plays well against the turnips sweetness and kale flavour. Ramsons provides an experience in a completely different level and an opportunity to bring your mind off the dandelion bitterness. The salad sets the stage for subsequent stations.

Dandelion: Weed from my own garden, cleaned and cut into suitable pieces. The flavor is both bitter, sweet and nutty.

Turnip: Petrowsky Gulia, Ohlsens Enke strain, Vangede 1948. Originally I got the seeds from the NordGen genebank, has now grown it for two generations. A nice yellow turnip. In autumn, the peel is pretty sharp tasting, like radish. To my surprise, here in March, it’s quite mild and there is no reason to tear off the peel.

Ramsons: Picked in the nearest wood a few years ago, sliced, fermented and stored in sealed glass in the refrigerator. Appearance, aroma and texture quite like freshly fermented ramsons. A strong aroma could be felt throughout the room, although there were only used very small amounts. When it comes to ramsons, fermentation intensifies and improves the garlic-like aroma. It does not take more than 5-10 minutes to pick what I use in a year. Ramsons has a fairly long picking period in April and May. Be sure to identify it properly, as some people get poisoned from mistakenly eating Lily of the Valley or Colchicum.

Oil: Siberian pine nut oil. An oil with a slight hint of resin. Many know the resin taste of Greek retsina wine.

Verjuice: The juice of unripe Rondo grapes from my own garden. To increase shelf life, I vinegar fermented the verjuice with kombucha / vinegar culture. In this way it contains both tartaric acid and acetic acid formed by fermentation and the acidic profile of the salad becomes more balanced. Wine variety Rondo sets far more grapes than it can mature. I have to thin out in the grapes anyway, and then it’s wonderful to use it for a gourmet product like verjuice.

 

 


Chopped cabbage with eggplant, oca leaves and victory onion
©Randi og Svend Sørensen

Station 2
Chopped cabbage with eggplant, oca leaves and victory onion

Danish use of raw cabbage has shocked all Russians I know. That we eat chopped white cabbage, without doing more of it, is totally hardcore for a Russian. They will either squeeze it with a little salt, or ferment it. At this station, we taste the Danish version, to remember it when we later taste the other versions. To balance the raw cabbage, I add acid in various forms: fermented eggplant, oca leaves with oxalic acid and the sour cream with victory onion. It is reminiscent of wild ramsons, and yet, not quite?

Cabbage: Purchased organic import from the Netherlands. The chopped cabbage is a standard winter salad in Denmark. It is included here for comparison of chopped white cabbage in later stations where it is respectively squeezed and fermented.

Eggplant: Grown outdoor in my own garden. Lactic acid fermented with garlic topsets (forgot the variety) and mild chilli, Medina. It is unusual to eat eggplant raw, but it’s fine when it is fermented – it can save a lot of CO2 :-). Fermentation seems like a long marinating, creating its own acidity and allowing flavour of garlic and mild chilli to penetrate the eggplants completely. If you have cold storage, either in a cold pantry or refrigerator, the various pickled vegetables are easy fast food, letting you balance a meal in a hurry.

Oca: I put my oca tubers for sprouting weeks before, to garnish with the fresh acid tasting clover like leaves. As oca is daylength sensitive, is almost impossible to grow big oca tubers in Denmark, but easy to make many small tubers. They store well in a paper bag at room temperature, and can be set to sprout fresh and neat leaves.

Victory onion: Brought from Irkutsk in Siberia. Victory onion, Allium victorialis, is a relative of ramsons. It is harvested from wild populations in the Siberian taiga, fermented and sold in marketplaces. In sour cream (smetana) it acts as a sauce or dip. Have as salted ramson potent aroma, slightly different, but clearly related. In my garden it grows at a slow pace like peonies. It is highly ornamental, a true perennial edimental.

 

 


Cider
©Randi og Svend Sørensen

Station 3
Cider from Filippa apples and seedling apples from the local wood

Time for a little drink to clean the mouth and refresh the taste buds.

Filippa: Fallen apples from my garden fermented into cider. The delicate Filippa taste is evidently in the cider

Apples from seed grown trees: Several places in the local wood are growing apple trees obviously grown from seed. Every one of them are unique. None of them are juicy like Filippa. I have the impression, that they didn’t really do anything for the cider, just being bulk. No problem, as I needed some apple juice to fill the fermentation tank.

The cider was started with a bottle of home made cider, to get fermentation started quickly. When the fermentation was ended, I bottled the cider with a little sugar to a get a light secondary fermentation. This carbonates the cider.

 

 


Grey peas, cabbage, cherry plums and Jerusalem artichoke
©Randi og Svend Sørensen

Station 4
Cooked grey peas, with squeezed white cabbage, fermented cherry plum and Urodny Jerusalem artichoke

Danish Seed Savers doing an EU founded project on rediscovering old peas and beans these years, calls for the use of grey peas. They need some crunch and acid from other elements for contrast.

Gray pea Lollandske rosiner: Old type of field peas grown by some seed savers. Soaked overnight with a teaspoon baking soda in the water. Then boiled two hours in fresh water with ½ teaspoon of baking soda. Since both yellow and grey peas are best second day, they were boiled up again and only this time I added a little salt. The peas do not disintegrate, but look like raisins in its own brown gravy. The taste is wonderful, although it is a very simple and inexpensive dish. Indeed, it’s also a healthy dish.

Squeezed white cabbage: Same cabbage, cut at the same setting on the mandoline. In this version sprinkled with a little salt and squeezed in a bowl. It takes approx. ½ hour, but you do not squeeze the cabbage continuously. Start squeezing it thoroughly for 2-3 minutes so that the cell walls burst. This releases enzymes, which starts a process that cures the cabbage to a gentle salat, even easier to digest. With 5-10 minutes intervals squeeze it again, and gradually it gets very juicy. The finished squeezed cabbage keeps for a week in the fridge. Make a big portion, and have fast healthy salad rest of the week.

Fermented cherry plums: Personally, I have had a slightly strained relationship with cherry plums. My mother was young during World War II. It taught her not to distinguish between plums and cherry plums. My childhood was therefore filled with cherry plums prepared by plum recipes, and it is not always successful. But a cherry plum tree in my garden has challenged me, and when I started fermenting cherry plums, it changed my view on cherry plums. When ripe and sweet, the fermentation will be alcoholic, due to the sugar content. This can probably become something wonderful too. But I pick cherry plums when they are still green but starting to turn yellow, and the sugar content still low. I wash them quickly in cold water, prick them with a fork, cover them in cold water with 5% salt and weigh them under the liquid surface with a plate. Then I store them at room temperature, to kick start the fermentation. After one to two weeks fermentation, I relocate them to a cooler setting (in the bottom of my pantry). Immediately before eating them, I soak them well in water to remove some of the salt. When young they taste very fresh and tangy, you can feel the carbon dioxide bubbles on the tongue. Later, after Christmas, the almond flavour of the kernels become more obvious, giving more depth to the flavour.

Jerusalem artichoke Urodny: This variety of Jerusalem artichoke is at the top in taste tests. And it is a clone everybody like, if they like Jerusalem artichokes at all. Here served sliced ​​raw.

 

 


Baked pumpkin with Jerusalem artichoke, pepper, pea shoots and lemon-grass
©Randi og Svend Sørensen

Station 5
Baked Trombo d’Albenga musk pumpkin with Dwarf Jerusalem artichoke, green pepper, Errindlev and Engelsk Sabel ESA pea shoots and lemon-grass

Baked pumpkin is soft mild carbohydrates. They must be pepped up and meet contrasts. Here in the form of crunchy Jerusalem artichoke and sour peppers. We will discover, if we like this Jerusalem artichoke, and compare it with the taste of Urodny from an earlier station. Do we experience a difference between the two varieties of pea shoots. We will experience the taste of vinegar fermented pepper.

Trombo d’Albenga musk pumpkin: Musk Pumpkin is heat-lover, and need a good summer to succeed. This northern Italian cultivar does seem to be among the best adapted. It can be used both as a summer squash and winter pumpkins. Here I cut the long massive neck in slices ​​and baked them in the oven, with a little salt and pepper. All pumpkins (and cucumbers) is high in potassium. This can be balanced with the sodium of salt.

Dwarf Jerusalem artichoke: Served sliced ​​raw. This clone divide people. Either you think it’s the world’s best Jerusalem artichoke or the worst of all! In taste tests, no one find it to be in the middle. Taste it before you grow it. The flavour is intense nutty with dark mineral tones.

Acetic fermented outdoor peppers: Green peppers are really good sliced and put in kombucha with spices and stored in the refrigerator. Here they were spiced with garlic topsets, a little mild chilli Medina, and a little verjuice. Kombucha should not be too young, but have developed a good acidity. In my kitchen, this corresponds to at least 2 weeks fermentation. You can also use kombucha fermented apple juice (apple vinegar). Acetic fermentation in this style differ from traditional pickling in that the acetic culture is alive. I find the method good for vegetables that will not fare well in a lactic fermentation. Typically dark green vegetables. Some will surely love lactic fermented green pepper, I just do not. In contrast, the red pepper is irresistible when lactic fermented.
In this method, the kombucha or vinegar is fermented already, allowing you to use herbs that would otherwise inhibit fermentation.

Errindlev pea: The peas were put to germinate about 14 days before, mostly in the greenhouse, but inside during cold periods. Shoots were served raw. They can also be used on top of a soup, in salads or in a wok. This variety is actually a grey pea, ie. a dry pea. But it works great as snow pea and pea shoots.

Engelsk Sabel ESA: Same treatment and serving as Errindlev pea. This variety is a tall snow pea, a strain developed by Ohlsens Enke, and preserved in Nordgen.

Lemon-grass: A piece of fresh leaf on top, to give a little fresh lemon aroma. People do not eat lemon grass, but chew on it, to enjoy the taste. This clone I once brought home from Thailand. In the summer, put it out in the kitchen garden, in winter, taken in to the house. This clone provides large lemon-grass stalks. When drying lemon-grass flavour is well preserved, as opposed to lemon balm, which lose flavour in drying.

 

 


Fermented cabbage with yacon, hawthorn ketchup and red pepper
©Randi og Svend Sørensen

Station 6
Fermented cabbage with yacon, hawthorn ketchup and red pepper

Here we come to the last cabbage, which is lactic fermented. As a German sauerkraut, without caraway, only slightly fermented, and still alive. We shall try yacon, which many have not yet tasted. In the wild end we taste the hawthorn ketchup. We also see the difference from the fermented vinegar peppers in the station before and this stations lactic fermented red pepper.

Fermented cabbage: Same cabbage and cut at the same setting on mandolin, as the chopped and squeezed cabbage from previous stations. This cabbage was treated like squeezed cabbage, and then immediately pressed into a glass, so the juice covered the cabbage. Close the lid and let ferment for one to two weeks. Every day unscrew and re-screw the lid, to relieve the pressure. It also gives a good feel for the fermentation progress. When you are satisfied with the result, store the fermented cabbage cool. The refrigerator keeps it fresh for a very long time, at higher temperatures the fermentation continues, depending on temperature. But there is plenty of time to use the fermented cabbage, even at not so cold temperatures. The fermentation can be varied endlessly with other vegetables, appropriate condiments and different slicing techniques. For those who do not eat fermented milk products, fermented vegetables provides the beneficial bacteria in your diet.

Yacon Morado: Grown by Kirsten Hedegaard.
Yacon is still a new vegetable in Denmark. Nutritionally, it resembles Jerusalem artichoke, but in terms of taste, one might call it the watermelon of root vegetables. Served raw, as here, it is sweet, juicy and crisp. Used in baked root vegetables it keeps its crispness quite a while before it finally starts to get soft. This variety is Morado, which has proven to be easy to grow in Denmark. It is grown almost as Dahlia. It is frost sensitive, so the tubers should be stored frost-free. They last well into spring by proper storage, and can thus still be used when Jerusalem artichoke season ends.

Hawthorn Ketchup: Hawthorn ketchup for today was made ​​with few spices to give a clearer impression of hawthorn berry taste. It is my experience that you have to taste the berries before you pick them. Pick from the bushes with best tasting berries.

Red open ground pepper: This is breeding material, that in a few more generations can become a new pepper cultivar. I grow it in the open air in each generation to make sure it will be a tough line. These ripe red peppers I have lactic fermented. Lactic fermentation brings out the best in mature red pepper (and maybe the worst in green?). I added garlic and verjuice to intensify the flavor. Verjuice make the pepper taste even more of pepper.

Station 7
Kombcha, ground ivy, Daubenton kale and Bergenia

Sorry, no photo. Again a little fluid on the palate. This time in the form of kombucha. For the brave, there is a version infused with fresh ground ivy. When you taste something unknown, it can feel reassuring holding something well known in the other hand. For this purpose I served some freshly cut Daubenton kale. The adventurer may also try a Bergenia tea.

Kombucha: Acetic fermented sweetened black tea. For this sample fermented five days, to preserve some of the sweetness. For other uses I ferment it two to four weeks.

Ground Ivy: A lovely spring flower and herb. I pluck it, and let it rest in kombucha some hours before filtering. Then, it can last a long time in a bottle in the refrigerator.
General considerations about kombucha and herbs: Many herbs can slow or change the kombucha fermentation. To prevent this I suggest you first do your kombucha, prepare a new batch, and only then infuse your herbs in kombucha. In this way, your mother culture is never exposed to the herbs antibiotic substances.

Daubenton kale: Small stemshoots served as a snack for kombucha. They were all eaten with eager, except what people took home as cuttings. This summer, there must be many small cabbages in many a seed saver garden derrived from this food workshop 🙂
Daubenton kale is a leafy brassica that never blooms, and therefore do not produce seeds. The are longlived, but can die off in cold winters. After a mild winter like this year, it is fantastic, filled with tiny tasty leaf shoots, which can either be eaten or used for cuttings.

Bergenia cordifolia: This plant I brought as tea leaves from my garden, and a Thermos of the fresh tea. Again I was surprised by the willingness to taste. In Siberia and Mongolia, it is not unusual to drink Bergenia tea. I recognize the taste. I was served this tea on several occasions in Siberia, without being told that this was different from not black tea. A mild black tea appearence, gentle flavour. You harvest this tea by picking the blackened leaves in february or march. Then the bitterness has leached out in winters rain. I washed the leaves in the kitchen sink before I wiped them dry and hanged for completely drying before storing.

 

 


“Cabello de ángel” and garlic cookies
©Randi og Svend Sørensen

Station 8
“Cabello de ángel” and garlic cookies

The end is near, and many wish some real sweet in the mouth now. A reward of jam on bread, which is probably not challenging, and the more challenging cookies with garlic.

“Cabello de ángel”: This was served on a small piece of bread. The jam was made from ​​a Siam seven year melon (Cucurbita ficifolia) by Kirsten Hedegaard, who also grew it in her garden. Siam is a very durable pumpkin that easily holds 1-2 years at room temperature, or maybe even 7 years, as the english name suggest? The flesh is white and stringy, black seeds. The flesh is also used in modern shark fin soup, saving the endangered sharks of the oceans. The seeds are also very tasty. I prefer them to C.pepo seeds.

Garlic Cookie: Oatmeal cookies baked with almonds, garlic and a little salt. There are several kinds of pastries with garlic that has been presented at our danish garlic competitions. It seems that to be good in pastries, the garlic must either be combined with chocolate or salt. Garlic in the sweet kitchen challenge our cultural concept of sweet and onion, but can easily be successful for those who are willing to accept the challenge

Station 9
Green and full ripe fennel seeds

Last station must open our eyes to the importance of harvest timing. And it must be the ending point for all previous stations. Inspiration comes from India, where I often got a little nip of anise seeds, when paing for a meal in a restaurant.

Fennel: At the end of the taste tests were two kinds of fennel seeds. Fully ripe seeds, which are also suitable for sowing. These seeds were harvested 2013. The second kind were harvested green in 2010. It was interesting that although both types taste of fennel, the green harvested, although old, had a finer and stronger flavour. The seeds were harvested in my garden, where I grow fennel as perennials

A big thanks to the talented women who helped me organize this workshop.

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Aiah Noack with Hablitzia

Naturplanteskolen, a new permaculture plant sale opened Friday. It is perhaps the smallest regular plant sale this year, but with a unique plant selection, some I have never before seen on the market. So far the opening hours are Fridays 12-20. Aiah Noack, who owns Nature nursery, is occupied with permaculture, natural plant breeding and art. For years she did plantbreeding in South Africa.

I came home with two cultivars of allåkerbär (Rubus × stellarcticus) (it takes two varieties for a good pollination), Vietnamese coriander (Persicaria odorata) and a large piece of weed fabric in the professional quality.

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Danish billberry, Vaccinium myrtillus

Typical for the exquisite plant selection, it is billberry of Danish origin, not Swedish or American, on sale. Not that there’s anything wrong with the others, but of the nursery sells the plants they think is best to grow in this environment, not what can be ordered in bulk from the Netherlands.

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Daubenton kale flowers rarely, allowing it to live for years

It was fantastic to see the beautiful Daubenton cabbages. Aiah got a cutting from me, which she has propagated and sell. This kale originates in France. It must be maintained by cuttings. I got a cutting of Stephen Barstow, who lives in Norway, near Trondheim. Great to see that it is now accessible to ordinary gardeners in the district.

Naturplanteskolen: http://naturplanteskolen.dk

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Hawberry (Crataegus laevigata og Crataegus monogyna)

There are plenty of hawberries on the commons, in hedgerows and scrubs. This year I couldn’t resist to pick some of them. When I got home, I found a recipe for ketchup based on hawthorn on Jonathan Wallace’s blog Self-sufficient in Suburbia.

Since I always have to try things a little different than stated in the recipe, I ended up making ketchup from: Hawthorn, kombucha, sugar, red wine vinegar, salt, garlic, black pepper, paprika, chilli and tarragon.

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Hawthorn Ketchup

The result was 4 glasses of hawberry ketchup. The taste is great, quite similar to tomato ketchup. This is the first time I’ve used hawberries. With this experience, I could not dream of growing tomatoes for ketchup. It is easier to go out on a nice day and pick the small berries, than it is to grow a similar number of tomatoes from seeds, taking care of them spring and summer.

The process is simple. Rinse the berries, cover them with half vinegar and half water (here I used kombucha and a little vinegar). Gently boil them 30 minutes and press them through a sieve. Kernels and stem residues remain in the sieve, out comes a beautiful red mass. The spices are added and the ketchup boiled again, then poured on scalded glasses. I immediately turn the glasses, to let the heat of the content pasteurize the lids.


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A couple of garlic braids

Garlic competition is over. It was an exciting day at Hvidløg & Vin (Garlic & Wine).

We were not that many, perhaps because of the weather this season. A table was put up in the barn, so we sat next to this years big garlic harvest drying in an air ventilated big box. Interesting to see how the professionals do the various phases of garlic cultivation. Great to be in a center for garlic!

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Bottom up ventilation of the garlic harvest

Lotte Ravn Lei gave a speech on their cultivation of garlic. There are some quite different considerations when growing garlic for so many people. For example they grow no hardneck garlic, as the high and rigid flower stalks make field operations more difficult. It is hard enough, because there are so many processes where each garlic must be handled by hand.
With regard to soil, they experience the clay soil to be ideal for garlic. They fertilize the soil with composted chicken manure in autumn, before they set the garlic. They tell that it is important to look after the soil, so that the earthworms thrive. They provide the best soil structure for the garlic.

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Which garlic is whitest?

Awards were amazing this year, as Ejner had donated his garlic drawings.

In the competition Kirsten had the largest garlic, an Estonian Red weighing 132 g.
Then came:
Inchelium Red 114 g (Kirsten)
Susan Delafield 111 g (Merete)
Transsylvanian 111 g (Merete)
Vigor 100 g (Kirsten)
Thermidrome 97 g (Lotte)
Bodil’s French Market 82 g (Søren) -Yes, I still haven’t grown the largest garlic 🙂

The whitest garlic was cultivated by Lotte. The variety is unknown as it was an off-type, differing a lot from the other garlics from same batch of garlic sets. Several of the garlic was probably just as white, but Lottes garlic had the largest white surface.

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The reddest garlic

Merete won with the darkest red garlic. There was no doubt. The variety is Estonian Red.

Finest garlic garlic braid was made by Ejner, the huge braid with many varieties, each labeled with variety name (see top photo). The biggest garlic braid we’ve seen yet our competition.

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Garlic cookie

We also had coffee and cake. Lotte provide drinks, while Kirsten had baked two delicious cakes. I brought some garlic cookies. You probably should be very crazy about garlic, to eat cake with garlic in. There were some who ate more than a single cookie. Myself, I’m pleased with my garlic cookies.

Thanks all you happy people who participated!


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Demonstration for free seeds in Brussels

Palm Sunday and the following Monday I was along with other seed savers in Brussel. We took part in Sunday’s and Monday’s demonstration and seed sharing.

The days were arranged in the network www.seed-sovereignty.org

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Never have I seen so many seeds being shared in a single day

Sunday was a cornucopia of seeds. There were free seeds to everybody. It was great to experience the warm generosity among people. The event was open to all, being a good opportunity to fill empty seed bags.

Personally, I was so overwhelmed that I hardly didn’t take any seeds. I mostly just got the seeds kind people put in my hand, as they said things like “these will be perfect for you”. Thank you, I’m sure they will.
One interesting portion seeds, however, I rejected. It was a Belgian “Princess” bean, which looked like our old Danish Princess Bean a lot. So much that I’m sure the two would be mixed up if I started sharing the Belgian with Danish seed savers. It could end up with the loss of the old Danish princess bean.
The Belgian did look as a good and exciting bean – I hope my rejection is understood.

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Belgian Kokopelli, Ariane

Most of us from Denmark stayed with Ariane, Marc and their 3 boys. They were incredibly hospitable – thank you. It gave an insight into the lives of an ordinary family in Belgium.

Ariane is active in the Belgian branch of Kokopelli, an association with origins in France, which has branches in many countries. Kokopelli challenge EU seeds directive by selling seeds via their website. Nice that some take a stand, breaking a way to make changes to the directive. There must not only be room for the industrial cultivars, but also be room for the wide variety of other cultivars, and room for small players in the market who might just do a little seed in their own fields, to sell through their website. Small sale should be legal. It would undoubtedly be an inexpensive way to preserve the old varieties. Maybe with a remark that they are not included in EU variety list, so we as consumers know what we are buying and not buying. Some consumers will no doubt see it as a sign of quality, if a variety is not listed in the catalog – personally I will not go that far. But I can not see how the variety list meet the need of ordinary gardeners. We private gardeners have different needs from farmers and commercial vegetable growers, and there is nothing wrong in this. We prefer a long harvest period, and many old varieties meet our needs.

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Steph and Patrick, Amsterdam

It was a wonderful mixture of people present. Activists, mountain farmers, seed savers, garden bloggers, scientists, and … well, I can not remember all. There were mainly people I did not know, but I also met people I know from the blogosphaere, like Steph and Patrick from Bifurcated Carrots, people I have swapped seeds with like Lieven David from de lusthofin Belgium.

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Demonstration for free seeds

The demonstration was colorful and happy. Click on the image, you can see more pictures on my Flickr photo page.

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Banner in Portuguese

It was a pleasure to hear (and read) many languages.

Support the free seeds (click), sign the petition

Read what Patrick writes

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Frøsamlerne (Danish seed savers) exchange list 2011

Again this year the list is long and interesting: Danish Seed Savers exchange list

See eg. the exciting Caucasian Spinach (Hablitzia tamnoides), originating from the Caucasus, and with history of cultivation in Scandinavia, mostly in manor gardens.

In my garden I have a few frozen pots with seedlings of Caucasian Spinach, from the seeds I got last year from a seed savers. When spring comes, I’ll transplant them near a tree or shrub in the shade, where it may otherwise be difficult to grow vegetables.
On facebook, there is now a Group, Friends of Hablitzia tamnoides, the Caucasian Spinach

Prinsesse
The old Danish Prinsesse bean

Prinsesse bean is a classic on the exchange list. It’s live Danish heritage, preserved by a vegetarian family for generations. 100 years ago it was a well-known cultivar, mentioned in gardening books as the earliest bean to grow. Now used mostly as dry bean, and then it’s a lovely name!
It is also spelled with c: Princesse

Do I need to translate the name: Princess it is in queens English 🙂

I was very happy, as I stumbled upon this site, giving the breeding work of Tom Wagner a new platform to connect with the world. Go visit them to see for yourself.

I met Tom Wagner in autumn 2009, when he gave plant breeding workshops in europe:  2009 Tom Wagner workshop

 

Seeds and tubers for food self-sufficiency and food security for backyard gardeners, homesteaders, and small farmers. This is where you will find seeds and tubers for crops like potatoes, wheat, triticale, and soy that you can actually live on. If you are looking for the former Tater-Mater seeds, look here.

New World Seeds & Tubers

Youtube is full of wonderful tutorials. The is the one you need right now, if your garden like mine is covered in deep snow. Please notice the accuracy in sowing depth!

Thanks a lot to KitchenGardeners.org (and go visit them!)

Hovedkoordinat-analyse af gamle danske ærter
Principal coordinate analysis of old Danish peas
(Click on picture to see more clearly)

Finally I finished writing a small report (in danish) about the old peas in the laboratory.
The report is located here: 2010 Resultat af ærteforsøg (DNA)
I had google translate this version, with a bit of my help: 2010 Pea results

When reading the results, so it is vital to understand that this investigation can determine whether some of the peas are genetically different, but can not determine whether they are genetically identical! We have investigated only some small bits of the total DNA.
The peas that look similar, are either identical or different, we do not know!

At the top is shown the principal coordinate analysis. It gives a good visual picture of the distance between our varieties. Some old Swedish varieties was blended in, as they have been studied previously. It gives some reference points to past studies.

The principal coordinate analysis is based of course, on some very concrete data, namely the length of the micro-satellites we tested.


Table. Displays length of each micro-satellite.

Micro-satellites we have measured have letter names. A9 – D21 – AC58 – C20 – AA5 – AC75.
We investigated 6 different, but one would not work, the second was identical in all varieties, and therefore uninteresting. The last four showed differences. Each micro-satellite was measured twice.

I end up concluding:

So there are varieties that we now know is different from all others in this sample.
There are also groups of varieties we should check for phenotype differences. It is a great advantage that it is now clearly delineated the varieties to be compared by cultivation experiments. There is probably also varieties that are identical with each other. It is not something that can be resolved by this trial, which is only suitable to detect differences in the 4 areas (micro-satellites), we got useful results from. Whether they are identical in many other micro-satellites, we can not know before we make a new attempt!

Read in the google translated report: 2010 Pea results

If you can take a bit more, this is the excel file we were sent after the course: Diversity analysis Pisum


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Pee shoots of heirloom varieties in the laboratory at Department of Agriculture and Ecology, Copenhagen University (Copyright Ingrid Nolde)

Last weekend I joined the Danish seed savers at the laboratory at the Department of Agriculture and Ecology (Copenhagen University). Gunter Backes and Jihad Orabi led us through a study of our heirloom pea collection. It was a fantastic and educational weekend!

In preparation, we had grown peas for 3-4 weeks, so they were ready for analysis. We had to use leaves that had not been given too much light. Chlorophyll can make it difficult to extract DNA material from the rest of the leaf tissue. For safety’s sake, we were two cultivating the same peas. It went well and we only used one set.

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Test tubes on ice (Copyright Ingrid Nolde)

We were divided into four groups. That way we could manage to work with all the varieties we had brought. After a brief introduction to laboratory work, to use gloves and gowns – take them off when you leave the lab, we started slicing the pea leafes.
Once cut, the natural enzymes in the leaves activates. To inhibit them, we set all tubes in a tray of ice. Millimetre small pieces were placed in the tubes, a solution of caustic soda were added and the tubes were boiled for 1 minute to denature the proteins. Then we blended the boiled leaves with the tip of a plastic stick and added a resolution that brought the pH down to a desired level. To separate DNA from the rest of the leaf broth, test tubes were centrifuged.

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Pea leaf broth bottom, DNA located at the top in the clear part of the liquid (Copyright Ingrid Nolde)

Now we could move the purified DNA into new tubes, still set in ice.
Then it was time to mix liquids with primer for the DNA fragments we searched for. We looked at 6 different micro satellites. Micro satellites are good to tell the difference between varieties of a species. A primer is the DNA sequence immediately before the micro satellite, leading the DNA strand to be cut at the desired location. We used a primer for each end of the micro satellite, because it must be “cut” at both ends. To the liquid is also added Taq DNA polymerase, an enzyme that replicates DNA at specific temperatures.

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We used pipettes for several hours. Even with multi pipettes there was plenty to do (Copyright Ingrid Nolde)

Next the test tubes are undergoing repeated temperature changes between
94 °C, where DNA strand separates
64-55 °C, where the primer binds to the DNA strand, and
72 °C, where Taq DNA polymerase copies the DNA strand from the primer and forward

By the repeated changes in temperature, we multiplied the selected micro satellite exponentially. The first cycle gave very little DNA material, but for each cycle, there was more copies. Just like reproduction of yeast in a bread dough.

Finally, we let an electric field pull micro satellites through a viscous liquid in capillary thin tubes. A dye was coupled to micro satellites, for a photocell at the end of capillaries to detect how quickly they passed by. Small micro satellites migrate faster through capillary tubes than larger.

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First presentation results

The photo shows the horizontal bands, each representing a pea variety. The small vertical bands are micro satellites. The red vertical band marks a scale, so we know how long the micro satellite strands are.
Jihad and Gunter did the analysis work Sunday morning, before we all met up – thank you!

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It was best to group the peas in 7 groups

The results will soon arrive in my Inbox. I look forward to delve deeper into the results. I might have a more to write about then.

Big thanks to both Gunter and Jihad for a learning and rewarding weekend 🙂

PS: I’m just a happy amateur. Feel free to comment if I’m wrong in some of the techicalities 🙂


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